Acidic Oligosaccharide Sugar Chain, a Marine-Derived Acidic Oligosaccharide, Inhibits the Cytotoxicity and Aggregation of Amyloid Beta Protein

Jinfeng Hu; Meiyu Geng; Jing Li; Xianliang Xin; Jia Wang; Minke Tang; Juntian Zhang; Xiao Zhang; Jian Ding

doi:10.1254/jphs.FPJ04004X

Full Papers

Acidic Oligosaccharide Sugar Chain, a Marine-Derived Acidic Oligosaccharide, Inhibits the Cytotoxicity and Aggregation of Amyloid Beta Protein

Jinfeng Hu, Meiyu Geng, Jing Li, Xianliang Xin, Jia Wang, Minke Tang, Juntian Zhang, Xiao Zhang, Jian Ding

Author information

Jinfeng Hu
Department of Pharmacology, Marine Drug and Food Institute, Ocean University of China
Meiyu Geng
Department of Pharmacology, Marine Drug and Food Institute, Ocean University of China
Jing Li
Department of Pharmacology, Marine Drug and Food Institute, Ocean University of China
Xianliang Xin
Department of Pharmacology, Marine Drug and Food Institute, Ocean University of China
Jia Wang
Department of Pharmacology, Marine Drug and Food Institute, Ocean University of China
Minke Tang
Institute of Materia Medica, Chinese Academy of Medical Sciences and Peking Union Medical College
Juntian Zhang
Institute of Materia Medica, Chinese Academy of Medical Sciences and Peking Union Medical College
Xiao Zhang
Division of Molecular Neuropharmacology, Department of Neurotec, Karolinska Institute, Huddinge University Hospital
Jian Ding
Shanghai Institute of Materia Medica, Shanghai Institute for Biological Sciences, Chinese Academy of Sciences

Keywords: acidic oligosaccharide sugar chain, amyloid beta protein, neurotoxicity, aggregation

JOURNAL FREE ACCESS

2004 Volume 95 Issue 2 Pages 248-255

DOI https://doi.org/10.1254/jphs.FPJ04004X

Details

Abstract

In this paper, we investigated interactions of the acidic oligosaccharide sugar chain (AOSC), derived from brown algae Echlonia kurome OKAM, with amyloid beta protein (Aβ). We observed that AOSC inhibited the toxicity induced by Aβ in both primarily cortical cells and the SH-SY5Y cell line. We also observed that AOSC inhibited the apoptosis induced by Aβ in SH-SY5Y by reducing the elevated level of intracellular calcium concentration ([Ca²⁺]_i) and suppressing the generation of reactive oxygen species. Surface plasmon resonance analysis demonstrated that AOSC had affinity for both freshly-dissolved Aβ and 48-h incubated Aβ. Furthermore, AOSC blocked the fibril formation of Aβ, which may be responsible for its anti-cytotoxic effects. Thus, our results indicate that AOSC might be a potentially therapeutic compound for Alzheimer’s disease.

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