Antioxidant Effects of Stereoisomers of N-Acetylcysteine (NAC), <sc><font size = "-2">L</font></sc>-NAC and <sc><font size = "-2">D</font></sc>-NAC, on Angiotensin II-Stimulated MAP Kinase Activation and Vascular Smooth Muscle Cell Proliferation

Moe Kyaw; Masanori Yoshizumi; Koichiro Tsuchiya; Yuki Izawa; Yasuhisa Kanematsu; Yoshiko Fujita; Nermin Ali; Keisuke Ishizawa; Aiko Yamauchi; Toshiaki Tamaki

doi:10.1254/jphs.SC0040061

Short Communications

Antioxidant Effects of Stereoisomers of N-Acetylcysteine (NAC), L-NAC and D-NAC, on Angiotensin II-Stimulated MAP Kinase Activation and Vascular Smooth Muscle Cell Proliferation

Moe Kyaw, Masanori Yoshizumi, Koichiro Tsuchiya, Yuki Izawa, Yasuhisa Kanematsu, Yoshiko Fujita, Nermin Ali, Keisuke Ishizawa, Aiko Yamauchi, Toshiaki Tamaki

Author information

Moe Kyaw
Department of Pharmacology, The University of Tokushima Graduate School of Medicine
Masanori Yoshizumi
Department of Pharmacology, The University of Tokushima Graduate School of Medicine
Koichiro Tsuchiya
Department of Clinical Pharmacology, Graduate School of Pharmaceutical Sciences, The University of Tokushima
Yuki Izawa
Department of Pharmacology, The University of Tokushima Graduate School of Medicine
Yasuhisa Kanematsu
Department of Pharmacology, The University of Tokushima Graduate School of Medicine
Yoshiko Fujita
Department of Pharmacology, The University of Tokushima Graduate School of Medicine
Nermin Ali
Department of Pharmacology, The University of Tokushima Graduate School of Medicine
Keisuke Ishizawa
Department of Pharmacology, The University of Tokushima Graduate School of Medicine
Aiko Yamauchi
Department of Pharmaceutical Information Science, Graduate School of Pharmaceutical Sciences, The University of Tokushima
Toshiaki Tamaki
Department of Pharmacology, The University of Tokushima Graduate School of Medicine

Keywords: N-acetylcysteine, glutathione, vascular smooth muscle cell

JOURNAL FREE ACCESS

2004 Volume 95 Issue 4 Pages 483-486

DOI https://doi.org/10.1254/jphs.SC0040061

View "Advance Publication" version (August 5, 2004).

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Abstract

We examined the effects of the stereoisomers of N-acetylcysteine (NAC), L-NAC and D-NAC, on cellular glutathione (GSH) concentration and whether NAC-regulated cellular GSH levels are directly associated with angiotensin II (Ang II)-induced intracellular signaling events in vascular smooth muscle cells (VSMC). Both L-NAC and D-NAC similarly increased intracellular GSH concentration. We found that L-NAC and D-NAC both inhibited Ang II-induced c-Jun N-terminal kinase and p38 mitogen-activated protein kinase activation and [³H]-thymidine incorporation in VSMC. Our present study indicates the comparable effects of NAC stereoisomers in regulating intracellular GSH and the redox-dependent intracellular signaling mechanisms in VSMC.

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