Production of transgenic Italian ryegrass expressing the betaine aldehyde dehydrogenase gene of zoysiagrass

Wataru Takahashi; Hideki Oishi; Masumi Ebina; Toshinori Komatsu; Tadashi Takamizo

doi:10.1270/jsbbs.60.279

Research papers

Production of transgenic Italian ryegrass expressing the betaine aldehyde dehydrogenase gene of zoysiagrass

Wataru Takahashi, Hideki Oishi, Masumi Ebina, Toshinori Komatsu, Tadashi Takamizo

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Keywords: environmental stress, forage grass, Lolium multiflorum, salinity tolerance, transformation

JOURNAL FREE ACCESS

2010 Volume 60 Issue 3 Pages 279-285

DOI https://doi.org/10.1270/jsbbs.60.279

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Abstract

Transgenic Italian ryegrass plants expressing the betaine aldehyde dehydrogenase gene of zoysiagrass (ZBD1) were produced via particle bombardment of embryogenic calluses. Although the growth of both non-transgenic and transgenic plants expressing ZBD1 was inhibited under salt stress, isolated tillers of the transgenic plants showed better re-rooting ability in vitro in the presence of 300 mM NaCl. Chlorophyll fluorescence values of the transgenic plants declined under salt stress, but were significantly higher than those of non-transgenic plants under salt stress, indicating the improved salt stress tolerance of transgenic plants.

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