Identification of Salt-Tolerant Sinorhizobium sp. Strain BL3 Membrane Proteins Based on Proteomics

Waraporn Tanthanuch; Panlada Tittabutr; Shabaz Mohammed; Rune Matthiesen; Montarop Yamabhai; Monchai Manassila; Ole Noerregaard Jensen; Nantakorn Boonkerd; Neung Teaumroong

doi:10.1264/jsme2.ME09185

Regular Papers

Identification of Salt-Tolerant Sinorhizobium sp. Strain BL3 Membrane Proteins Based on Proteomics

Waraporn Tanthanuch, Panlada Tittabutr, Shabaz Mohammed, Rune Matthiesen, Montarop Yamabhai, Monchai Manassila, Ole Noerregaard Jensen, Nantakorn Boonkerd, Neung Teaumroong

Author information

Waraporn Tanthanuch
Synchrotron Light Research Institute (Public Organization)
Panlada Tittabutr
School of Biotechnology, Institute of Agricultural Technology, Suranaree University of Technology
Shabaz Mohammed
Biomolecular Mass Spectrometry and Proteomics Group, Bijvoet Center for Biomolecular Research and Utrecht Institute for Pharmaceutical Sciences, Utrecht University
Rune Matthiesen
Bioinformatics Unit—CIC bioGUNE, Parque Tecnológico de Bizkaia Edificio
Montarop Yamabhai
School of Biotechnology, Institute of Agricultural Technology, Suranaree University of Technology
Monchai Manassila
School of Biotechnology, Institute of Agricultural Technology, Suranaree University of Technology
Ole Noerregaard Jensen
Department of Biochemistry and Molecular Biology, University of Southern Denmark
Nantakorn Boonkerd
School of Biotechnology, Institute of Agricultural Technology, Suranaree University of Technology
Neung Teaumroong
School of Biotechnology, Institute of Agricultural Technology, Suranaree University of Technology

Keywords: Sinorhizobium sp., membrane proteome analysis, mass tag, salt stress, strong cationic micro-columns

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Supplementary material

2010 Volume 25 Issue 4 Pages 275-280

DOI https://doi.org/10.1264/jsme2.ME09185

View "Advance Publication" version (July 24, 2010).

Details

Abstract

Sinorhizobium sp. BL3 is a salt-tolerant strain that can fix atmospheric nitrogen in symbiosis with leguminous host plants under salt-stress conditions. Since cell membranes are the first barrier to environmental change, it is interesting to explore the membrane proteins within this protective barrier under salt stress. The protein contents of membrane-enriched fractions obtained from BL3 were analyzed by nanoflow liquid chromatography interfaced with electrospray ionization tandem mass spectrometry. A total of 105 membrane proteins were identified. These proteins could be classified into 17 functional categories, the two biggest of which were energy production and conversion, and proteins not in clusters of orthologous groups (COGs). In addition, a comparative analysis of membrane proteins between salt-stressed and non-stressed BL3 cells was conducted using a membrane enrichment method and off-line SCX fractionation coupled to nanoLC-MS/MS. These techniques would be useful for further comparative analysis of membrane proteins that function in the response to environmental stress.

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