Detection of Betaproteobacteria inside the Mycelium of the Fungus Mortierella elongata

Yoshinori Sato; Kazuhiko Narisawa; Kazuto Tsuruta; Masafumi Umezu; Tomoyasu Nishizawa; Kenji Tanaka; Kaoru Yamaguchi; Masakazu Komatsuzaki; Hiroyuki Ohta

doi:10.1264/jsme2.ME10134

Short Communications

Detection of Betaproteobacteria inside the Mycelium of the Fungus Mortierella elongata

Yoshinori Sato, Kazuhiko Narisawa, Kazuto Tsuruta, Masafumi Umezu, Tomoyasu Nishizawa, Kenji Tanaka, Kaoru Yamaguchi, Masakazu Komatsuzaki, Hiroyuki Ohta

Author information

Yoshinori Sato
Institute for Global Change Adaptation Science, Ibaraki University
Kazuhiko Narisawa
Institute for Global Change Adaptation Science, Ibaraki University
Ibaraki University College of Agriculture
Kazuto Tsuruta
Ibaraki University College of Agriculture
Masafumi Umezu
Ibaraki University College of Agriculture
Tomoyasu Nishizawa
Ibaraki University College of Agriculture
Kenji Tanaka
Biological Resource Center (NBRC), Department of Biotechnology, National Institute of Technology and Evaluation
Kaoru Yamaguchi
Biological Resource Center (NBRC), Department of Biotechnology, National Institute of Technology and Evaluation
Masakazu Komatsuzaki
Institute for Global Change Adaptation Science, Ibaraki University
Ibaraki University College of Agriculture
Hiroyuki Ohta
Institute for Global Change Adaptation Science, Ibaraki University
Ibaraki University College of Agriculture

Keywords: endobacterium, N₂O production, Mortierella elongata, soil fungus

JOURNAL FREE ACCESS

2010 Volume 25 Issue 4 Pages 321-324

DOI https://doi.org/10.1264/jsme2.ME10134

View "Advance Publication" version (September 25, 2010).

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Abstract

Microscopic and molecular analyses showed the presence of endobacteria inside the mycelia of four out of twelve nitrous oxide (N₂O)-producing fungal isolates identified as Mortierella elongata. The 16S rRNA gene was successfully amplified with DNA extracted directly from the endobacterium-containing fungal strains and all sequences were related to that of Candidatus Glomeribacter gigasporarum in the family Burkholderiaceae. Bacterial endotoxin was detected in the endobacterium-positive fungal strains but only trace levels were found in endobacterium-negative strains. No significant relationship was found between the fungal N₂O-producing activity and the presence of endobacteria.

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