Microbes and Environments
Online ISSN : 1347-4405
Print ISSN : 1342-6311
ISSN-L : 1342-6311
Regular Papers
Enrichment of a Novel Marine Ammonia-Oxidizing Archaeon Obtained from Sand of an Eelgrass Zone
Naoki MatsutaniTatsunori NakagawaKyoko NakamuraReiji TakahashiKiyoshi YoshiharaTatsuaki Tokuyama
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Supplementary material

2011 Volume 26 Issue 1 Pages 23-29

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Abstract

Ammonia-oxidizing archaea (AOA) are generally cultivated at ammonium concentrations of less than 2 mM. The physiology and abundance in the environment of AOA suggest an important role in the nitrogen cycle. We report here a novel marine ammonia-oxidizing crenarchaeote, strain NM25 belonged to ‘Candidatus Nitrosopumilus’, that was enriched from coastal sand of an eelgrass zone and grew in a medium containing 15 mM ammonium at 30°C. A phylogenetic analysis based on the 16S rRNA gene revealed this crenarchaeote was related to the ammonia-oxidizing archaeon ‘Candidatus Nitrosopumilus maritimus’ strain SCM1, with 98.5% identity. The ammonia monooxygenase subunit A (amoA) gene of strain NM25 was less closely related to that of known cultivable AOA (>95%) and environmental clones (>97%). This finding suggests the existence of AOA adapted to high ammonium-containing environments.

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© Japanese Society of Microbial Ecology / Japanese Society of Soil Microbiology / Taiwan Society of Microbial Ecology
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