In clinical and experimental settings, the ¹³C breath test is performed to measure gastric emptying and has advantages of noninvasiveness and repeatability. We intended to apply the ¹³C breath test method to mice with an easy-to-handle solid test meal that is more physiological than liquid meals. Male ddY mice were trained to eat ¹³C-acetate-containing pellets as the solid test meal. Thirty minutes after administration of metoclopramide (0.3-3 mg/kg, p.o.) or atropine sulfate (0.3-3 mg/kg, i.p.), mice received the test meal and were placed in chambers. The ¹³CO₂ levels in the expired air were measured and the maximum concentration (C_max; ‰) and the time to reach the maximum concentration (T_max; min) were determined. Metoclopramide significantly and dose-dependently increased C_max and decreased T_max. On the other hand, atropine sulfate significantly and dose-dependently decreased C_max and increased T_max. The ¹³C-acetate breath test using a solid test meal is sensitive enough to detect both enhanced and delayed gastric emptying of the reference drugs.