In the past several years, peptide aptamers that bind to various inorganic materials have been isolated by using evolutional engineering methods including a peptide phage system. We have recently isolated a peptide aptamer, TBP-1, that electrostatically binds to the surface of titanium. So far we have investigated the binding of the peptide by scoring plaque forming units of the eluted phages. In this paper, we estimated the number of phages that bound to the surface of titanium through direct observation by AFM, and concluded that the estimations obtained both from biological method and AFM were consistent. We also performed the analysis of quarts crystal microbalance and viscoelasticity, and concluded that the TBP-1 displaying phage bound on the surface of titanium via its displayed peptide.