Experiments at a single molecular level provide spatial resolution and dynamic information for the study of biological processes under physiological environments. Here, we show several experimental results: 1) immobilization of a single-molecule in a microchannel using a combination of 3D nano-electrodes and an electrostatic force of dielectrophoresis; 2) drug delivery to a desired location, at which the molecules are immobilized, using a feature of laminar stream; 3) a microreactor for cell-free protein synthesis, as our continuous efforts to develop a micro platform for single-molecule research. For the time being, we are developing each components, however, a system including all the component will be developed in the future for an all-in-one type system to realize chip-based single-molecule analysis.