We review here several recent studies on protein patterning with microcontact printing (μCP), microfluidic networks (μFN), and dip-pen nanolithography (DPN). Microcontact printing (μCP) is a softolithographic technique and has been found to be appropriate for direct-printing with various proteins onto glass substrates at the monolayer level. However, little is known about the mechanism of the μCP process, in which the protein monolayer is transferred from the PDMS stamp to the substrate surface. Microfluidic network (μFN) features parallel microfluidic channels to make multi-color patterning with proteins, which allows us a high-throughput platform for multi-immunoassay named as “micromosaic immunoassay”. By combining the techniques of μFN and μCP, multi-color printing is possible with a single stamping. In DPN, relatively larger molecules such as immunoglobrin can be transferred from a cantilever probe to the substrate with nm-resolution. The transfer mechanism might be shared with μCP.