表面科学
Online ISSN : 1881-4743
Print ISSN : 0388-5321
ISSN-L : 0388-5321
論文
走査型プローブ顕微鏡応用によるゲノム解析技術の開発
杉山 滋塚本 和己山内 武志吉野 智之高橋 宏和桑崎 誠剛末次 克行生川 潤子山本 公子大谷 敏郎
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2009 年 30 巻 8 号 p. 427-432

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Recently, in the field of genome sequencing, next generation DNA sequencers are becoming available, and even the genome sequence analysis of a higher animal and/or a plant can be finished within a short period. However, the next generation DNA sequencers can only read relatively short sequence, which causes difficulty in the assemble step (i.e. reconstruction of original genomic sequence) and makes it labor and time consuming process and results in the long time requiring for gene isolation and breeding. When a target organism does not have enough genomic information, the assemble becomes much harder or not possible. This difficulty is arisen from the absence of positional index of the obtained nucleotide sequences by a whole genome shot-gun method. Therefore, we started the development of a new, scanning probe microscope based genome analysis method that can acquire the nucleotide sequences with positional indexes on the genome.

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この記事はクリエイティブ・コモンズ [表示 - 非営利 4.0 国際]ライセンスの下に提供されています。
https://creativecommons.org/licenses/by-nc/4.0/deed.ja
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