Substrate-supported phospholipid bilayers (SPBs) are being studied as models of cellular membranes. One of the important features of SPBs is the possibility of generating micropatterned membranes on the substrate, which enables the creation of designed arrays of biological materials for various applications. Building an assay platform that is capable of producing an array of model lipid bilayers that maintain many of the characteristics found in cellular membranes such as fluidity and biocompatibility and that allow for the investigation of protein/ligand and protein/membrane interactions in a multiplexed fashion is the target of our studies. It is reported that a new process for creating phospholipid bilayer microarrays (PLBMAs) with a prepatterned substrate by the use of poly [2-methacryloyloxyethyl phosphorylcholine (MPC)] (PMPC) brushes. The PLBMAs were shown to resist nonspecific protein adsorption and have a minimal amount of cross-reactivity. PLBMAs should have potential applications in many fields such as biosensing, drug discovery, proteomics, and clinical diagnostics.