Proteomics analysis of cardiac muscle from rats with peroxisomal proliferator-activated receptor α (PPARα) stimulation

Masafumi Miyazaki; Ippei Nakagawa; Shigeru Koga; Yasushi Kasahara; Matthew P. Patricelli

doi:10.2131/jts.35.131

Toxicogenomics/proteomics Report

Proteomics analysis of cardiac muscle from rats with peroxisomal proliferator-activated receptor α (PPARα) stimulation

Masafumi Miyazaki, Ippei Nakagawa, Shigeru Koga, Yasushi Kasahara, Matthew P. Patricelli

Author information

Keywords: Clofibrate, Fenofibrate, Heart, LC-MS/MS-based proteomics

JOURNAL FREE ACCESS

2010 Volume 35 Issue 1 Pages 131-135

DOI https://doi.org/10.2131/jts.35.131

Details

Abstract

To investigate peroxisomal proliferator-activated receptor α (PPARα) signal responses in heart muscle, we performed LC-MS/MS-based proteomics analysis of heart muscle from rats given fenofibrate or clofibrate. Fenofibrate increased the expression of ACAA2, DECR1, and ECH1 consistent with activation of PPARα. Fenofibrate and clofibrate reduced the expression of 10 and 12 proteins, respectively with the expression of ACSL1, SLC25A4, A1BG, HADHA, ATP2A2, BDH1, ETFDH, HADHB, and CPT2 being reduced in common with both of fibrate-treated groups. The approach adopted in this study provides an efficient method for monitoring global changes in protein expression.

Corresponding author

Register with J-STAGE for free!