26S rDNA-Based Phylogenetic Investigation of Japanese Cattle-Associated Prototheca zopfii Isolates

Yuji KISHIMOTO; Rui KANO; Haruhiko MARUYAMA; Masanobu ONOZAKI; Koichi MAKIMURA; Takaaki ITO; Kiyoshi MATSUBARA; Atsuhiko HASEGAWA; Hiroshi KAMATA

doi:10.1292/jvms.09-0115

Clinical Pathology

26S rDNA-Based Phylogenetic Investigation of Japanese Cattle-Associated Prototheca zopfii Isolates

Yuji KISHIMOTO, Rui KANO, Haruhiko MARUYAMA, Masanobu ONOZAKI, Koichi MAKIMURA, Takaaki ITO, Kiyoshi MATSUBARA, Atsuhiko HASEGAWA, Hiroshi KAMATA

Author information

Yuji KISHIMOTO
Department of Pathobiology, Nihon University School of Veterinary Medicine
Rui KANO
Department of Pathobiology, Nihon University School of Veterinary Medicine
Haruhiko MARUYAMA
Department of Pathobiology, Nihon University School of Veterinary Medicine
Masanobu ONOZAKI
Kanto Chemical Co. Inc.
Koichi MAKIMURA
Teikyo University Institute of Medical Mycology
Takaaki ITO
Livestock Medical Center, Aichi P.F.A.M.A.A
Kiyoshi MATSUBARA
Livestock Medical Center, Aichi P.F.A.M.A.A
Atsuhiko HASEGAWA
Teikyo University Institute of Medical Mycology
Hiroshi KAMATA
Department of Pathobiology, Nihon University School of Veterinary Medicine

Keywords: bovine Prototheca mastitis, phylogenetic investigation, Prototheca blaschkeae, rototheca zopfii

JOURNAL FREE ACCESS

2010 Volume 72 Issue 1 Pages 123-126

DOI https://doi.org/10.1292/jvms.09-0115

View "Advance Publication" version (November 13, 2009).

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Abstract

The study has been conducted to assess the applicability of partial 26S ribosomal DNA sequence for the phylogenic analysis of P.zopfii and to arrive relationships between specific genotype of P.zopfii and clinical mastitis. The phylogenetic analysis indicated that all genotype 2 isolates were grouped into the cluster of type strain of P.zopfii. Thus, all mastitis isolates in Japan were identified as P.zopfii genotype 2 and were independent of the cluster of the genotype 1 isolates. In one clinical case, a fecal isolate could not be identified by the 18S rDNA-based genotype specific PCR-assay and appeared to belong to a same cluster with the type strain of P. blaschkeae by 26S rDNA analysis. The isolate was the first isolation in Japan.

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