Immune Recovery Effects of Immunopotentiator from Pantoea agglomerans 1 (IP-PA1) on Low Antibody Productions in Response to Salmonella Enteritidis Vaccine and Sheep Red Blood Cells in Dexamethasone-Treated Stressed Chicken Models

Takehisa HEBISHIMA; Yasunobu MATSUMOTO; Gen-Ichiro SOMA; Chie KOHCHI; Gen WATANABE; Kazuyoshi TAYA; Yoshihiro HAYASHI; Yoshikazu HIROTA

doi:10.1292/jvms.09-0398

Avian Pathology

Immune Recovery Effects of Immunopotentiator from Pantoea agglomerans 1 (IP-PA1) on Low Antibody Productions in Response to Salmonella Enteritidis Vaccine and Sheep Red Blood Cells in Dexamethasone-Treated Stressed Chicken Models

Takehisa HEBISHIMA, Yasunobu MATSUMOTO, Gen-Ichiro SOMA, Chie KOHCHI, Gen WATANABE, Kazuyoshi TAYA, Yoshihiro HAYASHI, Yoshikazu HIROTA

Author information

Takehisa HEBISHIMA
Laboratory of Veterinary Physiology Division of Animal Life Science, Institute of Symbiotic Science and Technology, Graduate School, Tokyo University of Agriculture and Technology
The United Graduated School of Veterinary Sciences, Gifu University
Laboratory of Global Animal Resource Science, Graduate School of Agricultural and Life Sciences, The University of Tokyo
Yasunobu MATSUMOTO
Laboratory of Global Animal Resource Science, Graduate School of Agricultural and Life Sciences, The University of Tokyo
Gen-Ichiro SOMA
Institute for Health Sciences, Tokushima Bunri University
Department of Integrated and Holistic Immunology, Faculty of Medicine, Kagawa University
Chie KOHCHI
Institute for Health Sciences, Tokushima Bunri University
Department of Integrated and Holistic Immunology, Faculty of Medicine, Kagawa University
Gen WATANABE
Laboratory of Veterinary Physiology Division of Animal Life Science, Institute of Symbiotic Science and Technology, Graduate School, Tokyo University of Agriculture and Technology
The United Graduated School of Veterinary Sciences, Gifu University
Kazuyoshi TAYA
Laboratory of Veterinary Physiology Division of Animal Life Science, Institute of Symbiotic Science and Technology, Graduate School, Tokyo University of Agriculture and Technology
The United Graduated School of Veterinary Sciences, Gifu University
Yoshihiro HAYASHI
Laboratory of Global Animal Resource Science, Graduate School of Agricultural and Life Sciences, The University of Tokyo
Yoshikazu HIROTA
Laboratory of Veterinary Hygiene, Division of Animal Life Science, Institute of Symbiotic Science and Technology, Graduate School, Tokyo University of Agriculture and Technology
The United Graduated School of Veterinary Sciences, Gifu University

Keywords: chicken, immunomodulator, immunosuppression, IP-PA1, stress

JOURNAL FREE ACCESS

2010 Volume 72 Issue 4 Pages 435-442

DOI https://doi.org/10.1292/jvms.09-0398

View "Advance Publication" version (December 25, 2009).

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Abstract

Considering the usefulness of the immunopotentiator from Pantoea agglomerans 1 (IP-PA1), which is a purified lipopolysaccharide (LPS) derived from symbiotic gram-negative bacteria of food crops, in controlling immunosuppression in poultry husbandry, in this study, we examined its immune-recovery effects in dexamethasone-treated stressed chicken models. Three-week-old chickens daily administered 10 μg/kg of dexamethasone for 35 days to induce stress showed more whole body weight loss; relative thymic, bursal, and splenic weight losses; and decrease in the number of peripheral blood lymphocytes, as compared with the control chickens on day 35; the IP-PA1-pretreated, dexamethasone-treated chickens showed reduced weight losses. Five- to eight-week-old chickens administered 5 mg/kg of dexamethasone showed excessive apoptosis of thymic and bursal lymphocytes 24 hr after a single dexamethasone treatment; apoptosis was inhibited in the IP-PA1-pretreated, dexamethasone-treated chickens. Chickens daily administered 10 μg/kg of dexamethasone for 35 days and injected with commercial Salmonella Enteritidis (SE) vaccine or sheep red blood cells (SRBC) on days 7 and 21 showed about 8- or 2-fold lower antibody production in response to SE or SRBC, respectively, as compared with the control chickens on day 35; the antibody production in response to SE or SRBC was increased in the IP-PA1-pretreated, dexamethasone-treated chickens. These results indicate that IP-PA1 exerts inhibitory effects on dexamethasone-induced immunosuppression and that it may be useful in controlling immunosuppression in poultry husbandry.

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