Development of a New Selective Medium for Isolation of Methicillin-Resistant Staphylococcus aureus

Etsuko SAITO; Yoko KAKITA; Hiroyuki SHIMODA; Shizuka SHIMABUKURO; Keiichi SAEKI; Akira SHIMIZU; Junichi KAWANO

doi:10.1292/jvms.11-0021

Bacteriology

Development of a New Selective Medium for Isolation of Methicillin-Resistant Staphylococcus aureus

Etsuko SAITO, Yoko KAKITA ^†, Hiroyuki SHIMODA ^†, Shizuka SHIMABUKURO ^†, Keiichi SAEKI ^†, Akira SHIMIZU ^†, Junichi KAWANO ^†

Author information

Etsuko SAITO
Department of Bioresource and Agrobiosciences, Graduate School of Agricultural, Kobe University
Yoko KAKITA ^†
Laboratory of Microbiology and Immunology, Department of Bioresource Science Graduate School of Agriculture, Kobe University
Hiroyuki SHIMODA ^†
Laboratory of Microbiology and Immunology, Department of Bioresource Science Graduate School of Agriculture, Kobe University
Shizuka SHIMABUKURO ^†
Laboratory of Microbiology and Immunology, Department of Bioresource Science Graduate School of Agriculture, Kobe University
Keiichi SAEKI ^†
Laboratory of Microbiology and Immunology, Department of Bioresource Science Graduate School of Agriculture, Kobe University
Akira SHIMIZU ^†
Laboratory of Microbiology and Immunology, Department of Bioresource Science Graduate School of Agriculture, Kobe University
Junichi KAWANO ^†
Laboratory of Microbiology and Immunology, Department of Bioresource Science Graduate School of Agriculture, Kobe University
Author contributions

†These authors contributed equally: Yoko KAKITA, Hiroyuki SHIMODA, Shizuka SHIMABUKURO, Keiichi SAEKI, Akira SHIMIZU, Junichi KAWANO

Keywords: cefoperazone, ceftazidime, cephem, MRSA, selective medium

JOURNAL FREE ACCESS

2011 Volume 73 Issue 9 Pages 1195-1197

DOI https://doi.org/10.1292/jvms.11-0021

View "Advance Publication" version (April 28, 2011).

Details

Abstract

A new selective medium containing cephem antibiotics was developed for isolation of methicillin-resistant Staphylococcus aureus (MRSA). MRSA colonies on a medium containing ceftazidime (CAZ) were most easily identifiable and a medium containing cefoperazone (CPZ) was superior in suppressing the growth of other bacteria. With the medium containing a couple of CAZ and CPZ, MRSA and methicillin-resistant coagulase-negative staphylococci (MRCNS) were detected from 2 and 1 of 15 chicken meat samples respectively. The MRSA and MRCNS recovery test showed that the medium was effective for MRSA isolation, suppressing the growth of other bacteria efficiently. These results suggested that the medium containing a couple of CAZ and CPZ was useful for MRSA detection from foods and animals.

Corresponding author

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