Clonality Analysis of Various Hematopoietic Disorders in Cats Naturally Infected with Feline Leukemia Virus

Masaharu HISASUE; Kazuo NISHIGAKI; Hiromi KATAE; Kazuyo YURI; Takuya MIZUNO; Yasuhito FUJINO; Asuka SETOGUCHI; Atsuhiko HASEGAWA; Toshihiro WATARI; Kenichi MASUDA; Koichi OHNO; Hajime TSUJIMOTO

doi:10.1292/jvms.62.1059

Masaharu HISASUE, Kazuo NISHIGAKI, Hiromi KATAE, Kazuyo YURI, Takuya MIZUNO, Yasuhito FUJINO, Asuka SETOGUCHI, Atsuhiko HASEGAWA, Toshihiro WATARI, Kenichi MASUDA, Koichi OHNO, Hajime TSUJIMOTO

Author information

Masaharu HISASUE
Department of Veterinary Internal Medicine,Graduate School of Agricultural and Life Sciences,The University of Tokyo,Tokyo,Japan
Kazuo NISHIGAKI
Laboratory of Molecular Oncology,National Cancer Institute,Frederick,Maryland,U.S.A.
Hiromi KATAE
Research Laboratories,Animal Science Division,Dainippon Pharmaceutical Co.,Ltd,Osaka,Japan
Kazuyo YURI
Research Laboratories,Animal Science Division,Dainippon Pharmaceutical Co.,Ltd,Osaka,Japan
Takuya MIZUNO
Department of Veterinary Internal Medicine,Graduate School of Agricultural and Life Sciences,The University of Tokyo,Tokyo,Japan
Yasuhito FUJINO
Department of Veterinary Internal Medicine,Graduate School of Agricultural and Life Sciences,The University of Tokyo,Tokyo,Japan
Asuka SETOGUCHI
Department of Veterinary Internal Medicine,Graduate School of Agricultural and Life Sciences,The University of Tokyo,Tokyo,Japan
Atsuhiko HASEGAWA
Department of Pathobiology,Nihon University School of Veterinary Medicine,Kanagawa,Japan
Toshihiro WATARI
Laboratory of Comprehensive Veterinary Clinical Studies,Nihon University School of Veterinary Medicine,Kanagawa,Japan
Kenichi MASUDA
Department of Veterinary Internal Medicine,Graduate School of Agricultural and Life Sciences,The University of Tokyo,Tokyo,Japan
Koichi OHNO
Department of Veterinary Internal Medicine,Graduate School of Agricultural and Life Sciences,The University of Tokyo,Tokyo,Japan
Hajime TSUJIMOTO
Department of Veterinary Internal Medicine,Graduate School of Agricultural and Life Sciences,The University of Tokyo,Tokyo,Japan

Keywords: clonality, feline, feline leukemia virus, hematological disorder, myelodysplastic syndrome

JOURNAL FREE ACCESS

2000 Volume 62 Issue 10 Pages 1059-1065

DOI https://doi.org/10.1292/jvms.62.1059

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Abstract

The clonality analysis of the bone marrow cells was carried out by detecting the integrated proviruses of feline leukemia virus(FeLV)to understand the pathogenesis of FeLV−associated hematopoietic disorders in cats.Bone marrow cells from 4 cases with acute myeloid leukemia(AML), 9 cases with myelodysplastic syndromes(MDS), 2 cases with pure red cell aplasia(PRCA)and 3 healthy carriers infected with FeLV were subjected to Southern blot analyses using an exogenous FeLV probe.Clonal hematopoiesis was found in all the cases with AML and in 6 of the 9 cases with MDS, but not in the cases with both PRCA and healthy carriers infected with FeLV.In the 2 cases with MDS, it was thought that the same clones of the hematopoietic cells might proliferate before and after the progression of the disease irrespective of the changes of the hematological diagnoses by cytological examination.This study indicates that MDS in cats is a disease manifestation as a result of clonal proliferation of hematopoietic cells and can be recognized as a pre−leukemic state of AML.

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