2000 Volume 62 Issue 10 Pages 1109-1110
A quick method for sex determination of horses was developed.Simultaneous amplification of the equine sex−determining region of the Y chromosome gene(SRY)and amelogenin gene(AMEL)accomplished the determination of the presence of both the Y chromosome and SRY gene.In agarose gel electrophoresis, a normal stallion showed 1 SRY band and 3 AMEL(AMELX, AMELY, and AMELX/AMELY heteroduplex)bands, and a normal mare showed a single AMELX band.In XY−mares, 3 AMEL bands were detected as in a normal stallion, but no SRY band.The present method enables a quick diagnosis for XY−mare prior to cytogenetic analysis.
Journal of The Japanese Society of Veterinary Science
