Neurologia medico-chirurgica
Online ISSN : 1349-8029
Print ISSN : 0470-8105
ISSN-L : 0470-8105
Original Articles
Effects of N-Methyl-D-Aspartate, Glutamate, and Glycine on the Dorsal Column Axons of Neonatal Rat Spinal Cord: In Vitro Study
Masato MATSUMOTOTatsuya SASAKIHiroyasu NAGASHIMAEdward S. AHNWise YOUNGNamio KODAMA
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JOURNAL OPEN ACCESS

2005 Volume 45 Issue 2 Pages 73-81

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Abstract

The effects of N-methyl-D-aspartate (NMDA), glutamate, and glycine on the developmental axons of the neonatal rat spinal cord were investigated. Isolated dorsal column preparations from postnatal day (PN) 0 to 14 Long-Evans hooded rats (n = 119) were used in vitro. Compound action potentials (CAPs) were recorded from the cuneate and gracile fasciculi with a glass micropipette electrode. NMDA (100 μM) significantly increased CAP amplitude in PN 0-6 cords by 21.5 ± 9.2% (mean ± standard error of the mean, p < 0.001, n = 8) and in PN 7-14 cords by 6.7 ± 6.6% (p < 0.001, n = 10). NMDA (10 μM) significantly increased the CAP amplitude by 6.3 ± 2.9% in PN 0-6 cords (p < 0.01, n = 10). The increase of CAP amplitude induced by NMDA (100 μM) in PN 0-6 cords was significantly greater than that in PN 7-14 cords (p < 0.005). Glutamate (100 μM) significantly increased the CAP amplitude by 8.8 ± 8.1% in PN 0-6 cords (p < 0.001, n = 29) and 6.7 ± 7.5% in PN 7-14 cords (p < 0.01, n = 14), and glutamate (10 μM) significantly increased by 6.3 ± 2.9% in PN 0-6 cords (p < 0.01, n = 21). The amplitudes induced by glutamate (100 μM or 10 μM) did not significantly differ between PN 0-6 and PN 7-14 cords. Application of glycine (100 μM) did not significantly alter CAP amplitudes induced by NMDA (100 μM or 10 μM) and glutamate (100 μM or 10 μM). D(−)-2-amino-5-phosphonopentanoic acid (NMDA receptor antagonist) blocked the effects of NMDA and glutamate. These results suggest that NMDA receptor is present on afferent dorsal column axons and may modulate axonal excitability, especially during the 1st week after birth.

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© 2005 by The Japan Neurosurgical Society

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