Synthetic Seeds: A Novel Approach of In Vitro Plantlet Formation in Vasaka (Adhatoda vasica Nees.)

Yogeeta ANAND; Y. K. BANSAL

doi:10.5511/plantbiotechnology.19.159

Original Paper

Synthetic Seeds: A Novel Approach of In Vitro Plantlet Formation in Vasaka (Adhatoda vasica Nees.)

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Keywords: Adhatoda vacsica Nees, B5 medium, Calcium chloride, Encapsulation, Plantlet, Shoot bud, Sodium alginate, Synthetic seed, Tissue culture, Vasaka

JOURNAL FREE ACCESS

2002 Volume 19 Issue 3 Pages 159-162

DOI https://doi.org/10.5511/plantbiotechnology.19.159

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Abstract

Shoot buds of Adhatoda vasica Nees. isolated from multiple shoot cultures were encapsulated in 3% sodium-alginate with different gel matrices. Maximum conversion of the encapsulated shoot buds into plantlets was achieved on Gamborg’s B5 medium containing 4.65μM Kinetin and 50mgl^-1 Phloroglucinol and the plantlets were successfully grown in soil.

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