Formally, the authors carried out investigation of railway passenger awareness regarding hygiene and studied airborne microbes in railway stations. The results indicated that volatile compounds released from fungi partially caused odors in stations, and analysis of these compounds was performed. SPME (solid phase microextraction)-GCMS (gas chromatography-mass spectrometry) was adopted as a method of analysis for airborne volatile compounds, and analytical conditions were investigated. The technique was applied to the analysis of air in the field, and it was found that different results were obtained from separate locations where differing odors prevailed. In this way, it was confirmed that the method was suitable for field analysis.