The Journal of Antibiotics
Online ISSN : 1881-1469
Print ISSN : 0021-8820
ISSN-L : 0021-8820
Butirosin-biosynthetic Gene Cluster from Bacillus circulans
YASUMASA OTAHIDEYUKI TAMEGAIFUMITAKA KUDOHISAKO KURIKIAYUMI KOIKE-TAKESHITATADASHI EOUCHIKATSUMI KAKINUMA
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2000 Volume 53 Issue 10 Pages 1158-1167

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Abstract

Butirosin is an interesting 2-deoxystreptamine (DOS)-containing aminoglycoside antibiotic produced by non-actinomycete Bacilli. Recently we were successful in purification of 2-deoxy-scyllo-inosose synthase from butirosin-producer Bacillus circulans as the key enzyme for the biosynthesis of DOS, in cloning of the responsible gene (btrC), and in its overexpression in Escherichia coll. The present study involved gene-walking approach, which allowed us to find a gene cluster around btrC. The function of each gene was further investigated by gene disruption, and the disruptants of btrB, btrC, btrD and btrM showed no antibiotic producing activity. Therefore, the gene cluster found so far was determined to be a part of the butirosin biosynthetic gene cluster. Functions of some ORFs are also discussed in terms of butirosin biosynthesis on the basis of database search.

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© Japan Antibiotics Research Association
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