2001 Volume 54 Issue 5 Pages 434-440
The structures of the new antibiotics Streptocidins A-D were elucidated as cyclic decapeptides cyclo[L-Val1-L-Orn2-L-Leu3-D-Phe4-L-Pro5-L-Leu6-X7-L-Asn8-L-Gln9-X10] with X7-D-Trp (A, B, C) or D-Phe (D) and X10=L-Tyr (A), L-Trp (B, D), or D-Trp (G). The amino acid composition (including the configuration) of the substances was determined by chiralphase GC-MS of the hydrolysates. The sequences were established by EDMAN degradation following linearisation of the cyclic peptides upon treatment with LiAIH4. NMR spectroscopic studies of Streptocidins C and D confirmed the proposed sequences and provided conformational data which indicate a molecular topology of Streptocidins C and D similar to those of tyrocidine A and gramicidin S.
