Tannin acyl hydrolase (EC 3. 1. 1. 20) of Asp, oryzae No. 7 hydrolyzes tannic acid to glucose and gallic acid. The intermediate hydrolyzates are 1, 2, 3, 4, 6-pentagalloyl glucose, 2, 3, 4, 6-tetragalloyl glucose and two kinds of monogalloyl glucose.
The enzyme hydrolyzes ester compounds of gallic acid, but does not hydrolyze any other substrate analogues such as methyl-resorcyrate.
The enzyme reaction is inhibited competitively by substrate analogues which have phenolic hydroxyls with the exception that 2, 6-dihydroxy benzoic acid inhibits noncompetitively. Therefore the binding site of the enzyme may be able to react with any kind of phenolic hydroxyl, although the substrate forming a true ES-complex must be an ester compound of gallic acid.

This article cannot obtain the latest cited-by information.