Agricultural and Biological Chemistry
Online ISSN : 1881-1280
Print ISSN : 0002-1369
ISSN-L : 0002-1369
Cloning and Expression of the Creatinase Gene from Flavobacterium sp. U-188 in Escherichia coli
Yasuji KOYAMASatoshi KITAOHideko YAMAMOTO-OTAKEMasaru SUZUKIEiichi NAKANO
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JOURNAL FREE ACCESS

1990 Volume 54 Issue 6 Pages 1453-1457

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Abstract

The gene coding for creatinase (creatine amidinohydrolase, EC 3.5.3.3) was isolated from Flavobacterium sp. U-188. The primary structure of creatinase deduced from the nucleotide sequence showed a protein (molecular weight, 42, 651) composed of 378 amino acids. The creatinase gene was over-expressed in Escherichia coli under the control of the lac promoter and the amount of this enzyme was over 20 % of the soluble protein in the cell.

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© Japan Society for Bioscience, Biotechnology, and Agrochemistry
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