Bioscience, Biotechnology, and Biochemistry
Online ISSN : 1347-6947
Print ISSN : 0916-8451
Novel N-Terminal Sequence of a Glycine-rich Protein in the Aleurone Layer of Soybean Seeds
Miyoko MatsuiIsao ToyosawaMitsuru Fukuda
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JOURNAL FREE ACCESS

1994 Volume 58 Issue 10 Pages 1920-1922

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Abstract

Glycine-rich protein (GRP), a cell wall protein, was extracted with hot water from the aleurone layer of soybean seeds. GRP was purified by adsorption on DEAE-Sephadex, Sephadex G-100 gel chromatography, and anion exchange HPLC. The estimated molecular size of GRP was approximately 30kDa and GRP contained 59% glycine and 15% serine. The N-terminal amino acid sequence was a novel Gly-Gly-Gly-Gly-Gly-Gly-Gly-Gly-Gly-Gly-Gly-Gly-Gly-Gly-Gly-.

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© Japan Society for Bioscience, Biotechnology, and Agrochemistry
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