Gene Analysis of Trehalose-producing Enzymes from Hyperthermophilic Archaea in Sulfolobales

Kazuo KOBAYASHI; Masaru KATO; Yutaka MIURA; Masako KETTOKU; Toshihiro KOMEDA; Akihiro IWAMATSU

doi:10.1271/bbb.60.1720

Kazuo KOBAYASHI, Masaru KATO, Yutaka MIURA, Masako KETTOKU, Toshihiro KOMEDA, Akihiro IWAMATSU

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Keywords: trehalose, Sulfolobus, trehalose-producing enzyme, Southern analysis, genotype

JOURNAL FREE ACCESS

1996 Volume 60 Issue 10 Pages 1720-1723

DOI https://doi.org/10.1271/bbb.60.1720

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Abstract

The genes encoding new trehalose-producing enzymes from S. acidocaldarius ATCC33909 were cloned to analyze the distribution of these genes in Sulfolobales. Comparison of the amino acid sequences with S. solfataricus KM1 showed approximately 50% similarity. Southern analysis suggests that homologues of the trehalose-producing enzyme genes exist widely in Sulfolobales and strains in Sulfolobales were classified into three kinds of genotypes.

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