A shuttle vector, pBLES100, was constructed by cloning a Bifidobacterium longum plasmid and a gene encoding spectinomycin adenyltransferase AAD(9) from Enterococcus faecalis into the Escherichia coli vector pBR322. Stable transformants with this plasmid were obtained with an efficiency of 2.2×10⁴ transformants/μg DNA or 6.9×10^-5 transformants/cell/μg DNA under the optimal conditions of 10.0 kV/cm, 200Ω, and 25μF, using B. longum 105-A harvested at late log phase of growth.

This article cannot obtain the latest cited-by information.