1997 Volume 61 Issue 11 Pages 1949-1952
The nucleotide sequence of 1981 bp cDNA containing the entire coding region of a human placental fructose-6-phosphate, 2-kinase/fructose-2, 6-bisphosphatase was determined. The sequence encodes 469 amino acids and, based on homology to the rat testis enzyme, appears to be the testis-type isozyme expressed in placenta. The enzyme was expressed in Escherichia coli BL21 (DE3) by using a T7 RNA polymerase-based expression system and purified to homogeneity. The expressed enzyme was bifunctional with specific activities of 75 and 80 mU/mg of kinase and phosphatase, respectively. Kinetic parameters of the expressed enzyme are similar to those of the rat testis enzyme.
This article cannot obtain the latest cited-by information.