The Journal of Biochemistry
Online ISSN : 1756-2651
Print ISSN : 0021-924X
N-Terminal Sequence of Soybean β-Amylase
Bunzo MIKAMIKeiichi NOMURAYuhei MORITA
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1986 Volume 100 Issue 2 Pages 513-516

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Abstract

The blocked N-terminus and N-terminal sequence of soybean β-amylase were determined by analyzing the acidic peptides derived on peptic digestion of the enzyme. The acidic peptides were separated from the digest on a Dowex 50×2 column and purified by reversed phase-high performance liquid chromatography (RP-HPLC). The major acidic peptide, Pep-4, was a heptapeptide with a molecular weight of 766. Forty-eight hundredths mol acetyl group and 0.61mol acetyl-Ala per mol of Pep-4 were detected on RP-HPLC analysis. The N-terminal 9 amino acid sequence of soybean β-amylase was deduced to be acetyl-Ala-Thr-Ser-Asp-Ser-Asn-Met-(Gly-Leu) from the results of sequence analysis of Pep-4 and amino acid analysis of other acidic peptides.

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© The Japanese Biochemical Society
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