The Journal of Biochemistry
Online ISSN : 1756-2651
Print ISSN : 0021-924X
Presence of Phosphatidylcholine Hydroperoxide in Human Plasma
Teruo MiyazawaKeiichi YasudaKenshiro FujimotoTakashi Kaneda
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1988 Volume 103 Issue 5 Pages 744-746

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Abstract

A chemiluminescence-high performance liquid chromatography (CL-HPLC) system was newly developed and used for the hydroperoxide-specific determination of phosphatidyl-choline hydroperoxide (PCOOH) in human plasma. The method involves separation of phosphatidylcholine derivatives from plasma lipids by normal phase HPLC and subsequent detection of hydroperoxide-dependent chemiluminescence (CL) of PCOOH. CL was produced through luminol oxidation during the reaction of the hydroperoxide and cytochrome c-heme. The high specificity for the hydroperoxide allows the sensitive assaying of a large PCOOH range over a concentration range of 50-2, 000 pmol of hydroperoxide-O2. Using this method, the occurrence of PCOOH in normal human plasma was strongly suggested and was confirmed quantitatively.

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© The Japanese Biochemical Society
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