The Journal of Biochemistry
Online ISSN : 1756-2651
Print ISSN : 0021-924X
Identification of a Novel Unconventional Myosin from Scallop Mantle Tissue
Yasushi HasegawaTakahiro Araki
Author information
JOURNAL FREE ACCESS

2002 Volume 131 Issue 1 Pages 113-119

Details
Abstract

We isolated a cDNA encoding a novel unconventional myosin from scallop mantle tissue (sc-allop un-conventional m-yosin: ScunM) and determined the nucleotide sequence. It comprises 2, 739 bp with 5' and 3'-noncoding sequences and has an open reading frame of 2, 334 bp that encodes 778 amino acids. While ScunM has a motor domain and a short tail domain without having light chain-binding IQ motifs like myosin XIV, the deduced amino acid sequence exhibits low homology, 30-36%, to known myosins. Phylogenetic analysis of the motor domain suggested that ScunM belongs to a novel unconventional myosin class. ScunM has an insertion of 67 amino acids in the putative actin-binding site (loop2 site). Western blot analysis with an antibody produced against the N-terminal region revealed that ScunM was strongly expressed in the mantle and mantle pallial cell layer of scallop.

Content from these authors
© The Japanese Biochemical Society
Previous article Next article
feedback
Top