The Journal of Biochemistry
Online ISSN : 1756-2651
Print ISSN : 0021-924X
Characterization of Cell Lines Stably Expressing Human Normal or Mutated EGFP-Tagged MC4R
Antonine BlondetMabrouka DoghmanMohamed RachedPhilippe DurandMartine BégeotDanielle Naville
Author information
JOURNAL FREE ACCESS

2004 Volume 135 Issue 4 Pages 541-546

Details
Abstract

The melanocortin receptor type 4 (MC4-R) is involved in food intake and represents a potential target for the treatment of some forms of obesity. The fluorescent protein EGFP was fused to the wild-type or mutated coding sequence of the human MC4-R. After transfection in HEK 293, clones stably expressing hMC4-R-EGFP were selected. Wild-type chimeric hMC4-R was well addressed to the cell membrane as demonstrated using confocal microscopy and displayed the same pharmacological characteristics as native hMC4R. NDP-αMSH induced a time-dependent internalization of MC4-R that was partially prevented by AgRP. The two mutated chimeric receptors studied here (CTCT deleted and C271A) showed a high alteration of their response to ligand and were retained inside the cells. In conclusion, we have developed a model of clones stably expressing EGFP-tagged-hMC4-R. This is the only such model available to date and it provides a useful tool to follow the trafficking of MC4-R inside living cells.

Content from these authors

This article cannot obtain the latest cited-by information.

© The Japanese Biochemical Society
Previous article Next article
feedback
Top