1. N-Acetyl-β-D-glucosaminidase [EC 3. 2. 1. 30] was isolated from Takadiastase Sankyo by means of several purification methods.
2. The purified enzyme was shown to be homogeneous by disc electrophoresis and ultracentrifugation.
3. The molecular weight of the enzyme was determined to be 140, 000-146, 000 by equilibrium ultracentrifugation.
4. The enzyme was a glycoprotein, and the amino acid and sugar composition of this enzyme was determined.
5. N-Acetyl-β-D-galactosaminidase activity was found in water extract of Takadiastase and the two activities were not separated from each other.
6. The K_m and pH optimum of the enzyme were determined by using p-nitrophenyl and phenyl N-acetyl-β-D-glucosaminides and phenyl N-acetyl-β-D-galactosaminide as substrates.