The Journal of Biochemistry
Online ISSN : 1756-2651
Print ISSN : 0021-924X
Branched-Chain Amino Acid Aminotransferase of Escherichia coli: Nucleotide Sequence of the ilvE Gene and the Deduced Amino Acid Sequence
Seiki KURAMITSUTomoko OGAWAHideyuki OGAWAHiroyuki KAGAMIYAMA
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JOURNAL FREE ACCESS

1985 Volume 97 Issue 4 Pages 993-999

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Abstract

The ilvE gene of the Escherichia coli K-12 ilvGEDA operon, which encodes branchedchain amino acid aminotransferase [EC 2. 6. 1. 42], was cloned. The nucleotide sequence of 1.5 kilobase pairs containing the gene was determined. The coding region of the ilvE gene contained 927 nucleotide residues and could encode 309 amino acid residues. The predicted molecular weight, amino acid composition and the sequence of the N-terminal 15 residues agreed with the enzyme data reported previously (Lee-Peng, F. -C., et al. (1979) J. Bacteriol. 139, 339-345). From the deduced amino acid sequence, the secondary structure was predicted.

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© The Japanese Biochemical Society
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