Comparative Study on Amino Acid Sequences of Kunitz-Type Soybean Trypsin Inhibitors, Tia, Tib, and Tic

Seung-Ho KIM; Saburo HARA; Sumihiro HASE; Tokuji IKENAKA; Hiroko TODA; Keisuke KITAMURA; Norihiko KAIZUMA

Comparative Study on Amino Acid Sequences of Kunitz-Type Soybean Trypsin Inhibitors, Ti^a, Ti^b, and Ti^c

Seung-Ho KIM, Saburo HARA, Sumihiro HASE, Tokuji IKENAKA, Hiroko TODA, Keisuke KITAMURA, Norihiko KAIZUMA

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JOURNAL FREE ACCESS

1985 Volume 98 Issue 2 Pages 435-448

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Abstract

The amino acid sequences of three variants of the Kunitz-type trypsin inhibitors, Ti^a, Ti^b, and Ti^c, obtained from some cultivars of soybean were determined by conventional methods. All three inhibitors consisted of 181 amino acid residues. The differences in the amino acid sequences are as follows:
Ti^a E¹² G⁵⁵ Y⁶² H⁷¹ S⁷⁴ M¹¹⁴ L¹²⁰ P¹³⁷ L¹⁷⁶
Ti^b S F N R V I T V
Tic E
The amino acid sequences of Pro (60) -Ser (61) and Asp (154) -Asp (155) -Gly (156) His (157) of Ti^a reported previously (Koide & Ikenaka (1973) Eur. J. Biochem. 32, 417-431) were amended to Ser (60) -Pro (61) and His (154) -Asp-Asp-Gly (157), respectively.

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