The nutrient intake and iron status of female collegiate lacrosse players were investigated. The subjects were 12 players from one of the top teams in the Kyushu district. Six subjects (the anemia group) were classified into two blood iron levels: five subjects had iron depletion anemia, and one had ex-latent iron depletion. The other six subjects were normal in their iron status (the normal group). The anemia group had a lower iron intake than the normal group. The energy intake for both groups did not reach the estimated energy requirement for activity factor level I, although the protein intake met the estimated protein requirement. We recommend providing nutritional counseling to the players.