Modulation of Angiotensin II Type 1 Receptor mRNA Expression in Human Blood Cells Comparison of Platelets and Mononuclear Leucocytes

HIROMICHI SUZUKI; HIROTAKA SHIBATA; MAROHITO MURAKAMI; ATSUHISA SATO; MAREO NAITO; ATSUHIRO ICHIHARA; AKIRA MATSUMOTO; GOZOH TSUJIMOTO; AKIRA HIRASAWA; KUNIKO HORIE; TAKAO SARUTA

doi:10.1507/endocrj.42.15

Modulation of Angiotensin II Type 1 Receptor mRNA Expression in Human Blood Cells

Comparison of Platelets and Mononuclear Leucocytes

HIROMICHI SUZUKI, HIROTAKA SHIBATA, MAROHITO MURAKAMI, ATSUHISA SATO, MAREO NAITO, ATSUHIRO ICHIHARA, AKIRA MATSUMOTO, GOZOH TSUJIMOTO, AKIRA HIRASAWA, KUNIKO HORIE, TAKAO SARUTA

Author information

HIROMICHI SUZUKI
Department of Internal Medicine, School of Medicine, Keio University
HIROTAKA SHIBATA
Department of Internal Medicine, School of Medicine, Keio University
MAROHITO MURAKAMI
Department of Internal Medicine, School of Medicine, Keio University
ATSUHISA SATO
Department of Internal Medicine, School of Medicine, Keio University
MAREO NAITO
Department of Internal Medicine, School of Medicine, Keio University
ATSUHIRO ICHIHARA
Department of Internal Medicine, School of Medicine, Keio University
AKIRA MATSUMOTO
Department of Internal Medicine, School of Medicine, Keio University
GOZOH TSUJIMOTO
Department of Pediatric Pharmacology, National Children's Medical Research Center
AKIRA HIRASAWA
Department of Pediatric Pharmacology, National Children's Medical Research Center
KUNIKO HORIE
Department of Pediatric Pharmacology, National Children's Medical Research Center
TAKAO SARUTA
Department of Internal Medicine, School of Medicine, Keio University

Keywords: Angiotensin II type I receptor mRNA, Renin-angiotensin system, Platelets, Mononuclear leucocytes, Reverse transcription-polymerase chain reaction (RT-PCR)

JOURNAL FREE ACCESS

1995 Volume 42 Issue 1 Pages 15-22

DOI https://doi.org/10.1507/endocrj.42.15

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Abstract

The objective of the present study was to quantify the expression of angiotensin II type 1 (AT1) receptor transcripts in human blood cells-platelets and mononuclear leucocytes-from 10 normal healthy volunteers during the alterations in the renin-angiotensin system. A quantitative assay employing reverse transcription-polymerase chain reaction (RT-PCR) was utilized. Oral administration of furosemide, 40mg for 2 days, under mild salt restriction (50mEq NaCl/day) for 6 days stimulated the rennin-angiotensin system resulting in significant increases in plasma renin activity (PRA) (1.84±0.12vs. 1.05± 0.17ng/l/s; P<0.01), plasma angiotensin II concentration, and plasma aldosterone concentration (PAC). The ratio of AT1 receptor mRNA to glyceraldehyde-3-phosphate dehydrogenase (GAPDH) mRNA expression in mononuclear leucocytes was significantly (P<0.05) increased from the basal level (0.49±0.05 vs. 0.29±0.03) (P<0.01), while in platelets these changes were opposite (0.11±0.05vs. 0.25±0.05) (P<0.01). Compared to these significant changes, salt loading (200mEq NaCl/day) for 6 days decreased PRA(0.49±0.10vs. 1.05±0.17ng/l/s; P<0.01) and induced the opposite changes in the ratio of AT1 receptor/GAPDH mRNA. These data suggest that AT1 receptors in human blood cells may be of two different types-platelets and mononuclear leucocytes.

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