Immunological Study of Anti-M2 in Antimitochondrial Antibody-Negative Primary Biliary Cirrhosis

Noriyuki KITAMI; Toshiyuki KOMADA; Hideo ISHII; Hidetake SHIMIZU; Hiroyasu ADACHI; Yasushi YAMAGUCHI; Tsuneo KITAMURA; Hirosumi OIDE; Akihisa MIYAZAKI; Masakuni ISHIKAWA; Keiichiro ENDO; Sumio WATANABE; Yukio YOKOI; Nobuhiro SATO

doi:10.2169/internalmedicine.34.496

Noriyuki KITAMI, Toshiyuki KOMADA, Hideo ISHII, Hidetake SHIMIZU, Hiroyasu ADACHI, Yasushi YAMAGUCHI, Tsuneo KITAMURA, Hirosumi OIDE, Akihisa MIYAZAKI, Masakuni ISHIKAWA, Keiichiro ENDO, Sumio WATANABE, Yukio YOKOI, Nobuhiro SATO

Author information

Noriyuki KITAMI
the Department of Gastroenterology, Juntendo University School of Medicine
Toshiyuki KOMADA
the Department of Gastroenterology, Juntendo University School of Medicine
Hideo ISHII
the Department of Gastroenterology, Juntendo University School of Medicine
Hidetake SHIMIZU
the Department of Gastroenterology, Juntendo University School of Medicine
Hiroyasu ADACHI
the Department of Gastroenterology, Juntendo University School of Medicine
Yasushi YAMAGUCHI
the Department of Gastroenterology, Juntendo University School of Medicine
Tsuneo KITAMURA
the Department of Gastroenterology, Juntendo University School of Medicine
Hirosumi OIDE
the Department of Gastroenterology, Juntendo University School of Medicine
Akihisa MIYAZAKI
the Department of Gastroenterology, Juntendo University School of Medicine
Masakuni ISHIKAWA
the Department of Gastroenterology, Juntendo University School of Medicine
Keiichiro ENDO
the Department of Gastroenterology, Juntendo University School of Medicine
Sumio WATANABE
the Department of Gastroenterology, Juntendo University School of Medicine
Yukio YOKOI
the Department of Gastroenterology, Juntendo University School of Medicine
Nobuhiro SATO
the Department of Gastroenterology, Juntendo University School of Medicine

Keywords: M2 protein, pyruvate dehydrogenase complex, protein X, antinuclear antibody, smooth muscle antibody, anticentromere antibody

JOURNAL FREE ACCESS

1995 Volume 34 Issue 6 Pages 496-501

DOI https://doi.org/10.2169/internalmedicine.34.496

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Abstract

We investigated the specificity of various autoantibodies in antimitochondrial antibody (AMA)-negative patients with primary biliary cirrhosis (PBC). We examined sera from 144 patients with PBC, 17 of whom were AMA negative by indirect immunofluorescence. The AMA-negative group showed a significantly higher positivity for smooth muscle antibody, but not for antinuclear antibody, as compared with the AMA-positive group. IgG class anti-PDH by enzyme-linked immunosorbent assay (ELIS A) were detected in 13 % of the AM A-negative group. However, all PBC patients showed positive IgG, IgA, and/or IgM class anti-M2 to the four M2 proteins by immunoblotting. These results suggest that the detection of IgG and IgM class anti-PDH and that of antibodies to the four M2 proteins increases the positivity of this ELISA method, and that detection of IgG, IgA, and IgM class anti-M2 to the four M2 proteins by immunoblotting is useful in diagnosing AMA-negative patients with PBC.
(Internal Medicine 34: 496-501, 1995)

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