1983 Volume 49 Issue 5 Pages 670-675
Purification of rice ragged stunt virus (RRSV) was improved by using Triton X-100 as a detergent, adding MgCl2 in suspension buffers and using histidine buffer in the latter step of the procedure. The purified virus particles were about 55nm in diameter and highly infectious to rice seedlings, inoculated via vector insects (Nilaparvata lugens). Ten double stranded ribonucleic acid (RNA) segments with approximately equimolar amounts, including newly found two segments, were found in purified RRSV. A total molecular weight of the RNA was 22.91×106 based on the standards doveloped by Galinski et al.
