Anti-HCV (c100-3, Abbott 2nd, N-14 and AR-142) were assayed in the sera of 531 medical employees (196 physicians, 300 nurses and 35 technologists). Positive rate of anti-HCV were 1.1% (c100-3), 0.9% (Abbott 2nd), 0.6% (N-14) and 0.8% (AR-142) respectively. Anti-HCV was positive by at least one of these 4 tests in 11 cases (2.1%). Only 3 cases out of these 11 were proved positive for HCV-RNA. These data indicate that medical employees do not belong to the high-risk group of HCV infection.