Studies on the distribution of trace element in biological samples have been carried out to investigate physiological functions of these elements and to establish a method for monitoring environmental pollutions. Particle Induced X-ray Emission (PIXE) analysis is available for this purpose, since PIXE is sensitive enough to detect many trace elements simultaneously and without any separation processes using proton beam scanning even with a small sample. In this paper, we report spatial distributions of various trace elements in several human tissues and foods, and discuss their environmental significance.