The emulsionized Methyl-CCNU (Me-CCNU-E) was injected endoscopically into normal canine stomachs. The emulsion was detected in the submucosal layer of the stomach wall and regional lymphnodes histologically by Oil-red-O stain. Using the Me-CCNU concentrated in higher than 20mg/ml, ulceration was made in the gastric mucosa where the emulsion was injected. But in case of the concentration of Me-CCNU was lower than 10mg/ml, no compicated lesion was found.
Stomach cancers were induced in two Beagle dogs experimentally by N-ethyl-N'-nitro-N-nitrosoguanidine (ENNG), and Me-CCNU-E was injected directly into the tumors endoscopically.
One dog had advanced stomach cancer which was classified into Borrmann type III. Me-CCNU (10mg/ml) was injected into the tumor 9 times within 13 weeks in total dose of Me-CCNU, 130mg.
At the 17 weeks after the initial injection, the dog was sacrified and examined histologically. The cancer was not reduced, but degenerative findings were observed histologically in the tumor where the emulsion was injected. Regional lymphnode metastasis was not found. In the other dog, a II a + II c shaped advanced cancer and two II c shaped early cancers had been induced by ENNG. Total dose of 120mg of Me-CCNU-E (20mg/ml) was also injected into those tumors. At the 12 weeks after the treatment, this dog died for a perforation of the stomach.
On the histological examination, cancer nests were still remained, however most of the tumors were vanished distinctly.
It has been reported that the endoscopic injection of anticancer agents to the stomach cancer is useful method for the treatment of stomach cancer in the cases of high aged patients and poor risk patients. Through this experiment, it was indicated that the treatment of anticancer agents with emulsion was more effective to cure not only the cancer but the lymphnode metastasis.