This study focuses on assessment of the spatial transferability of parameters of a physically based distributed hydrological model, Blockwise use of TOPMODEL with Muskingum-Cunge routing (BTOPMC), to simulate the runoff in different physiographic regions of Nepal. The model was calibrated and validated using different time series data of six basins located in different physiographic regions of Nepal. The model parameters obtained after calibration from six basins were averaged and then applied in other two data limited basins for the jackknife validation of regionalization scheme. The model performance was evaluated by Nash-Sutcliffe efficiency, volume bias and qualitative comparison of observed and simulated hydrograph. The result of model calibration and validation in six basins shows that the model performs well in simulating runoff volume and capturing the seasonal and annual variations. Similarly, the result of model parameter transfer shows that the BTOPMC model parameters can be used to simulate the runoff components in ungauged basins.