A FEMALE PATIENT WITH POST-TRANSFUSION GRAFT-VERSUS-HOST DISEASE -USEFULNESS OF THE POLYMERASE CHAIN REACTION (PCR) USING PRIMERS TO SEX DETERMINING Y-

Hitoshi OHISHI; Norio OKAMURA; Takeshi YAMAZAKI; Kohichiro YORIMITSU; Hiroy TOHNO; Tomohide TAKAHASHI; Youichi NAKAMURA; Seigo TAKANO; Shigetomi IWAI; Takashi TANAKA; Satoshi HAYAKAWA; Masumi BABA

doi:10.3919/ringe1963.56.708

Hitoshi OHISHI, Norio OKAMURA, Takeshi YAMAZAKI, Kohichiro YORIMITSU, Hiroy TOHNO, Tomohide TAKAHASHI, Youichi NAKAMURA, Seigo TAKANO, Shigetomi IWAI, Takashi TANAKA, Satoshi HAYAKAWA, Masumi BABA

Author information

Hitoshi OHISHI
Departments of Surgery, Choshi City General Hospital
Norio OKAMURA
Departments of Surgery, Choshi City General Hospital
Takeshi YAMAZAKI
Departments of Surgery, Choshi City General Hospital
Kohichiro YORIMITSU
Departments of Surgery, Choshi City General Hospital
Hiroy TOHNO
Departments of Internal Medicine, Choshi City General Hospital
Tomohide TAKAHASHI
Third Department of Surgery, Nihon University School of Medicine
Youichi NAKAMURA
Third Department of Surgery, Nihon University School of Medicine
Seigo TAKANO
Third Department of Surgery, Nihon University School of Medicine
Shigetomi IWAI
Third Department of Surgery, Nihon University School of Medicine
Takashi TANAKA
Third Department of Surgery, Nihon University School of Medicine
Satoshi HAYAKAWA
Obstetrics and Gynecology, Nihon University School of Medicine
Masumi BABA
First Department of Internal Medicine, Nihon University School of Medicine

Keywords: transfusion associated graftversus-host disease (TA-GVHD), polymerasechain reaction (PCR), sex-determining region Y (SRY)

JOURNAL FREE ACCESS

1995 Volume 56 Issue 4 Pages 708-712

DOI https://doi.org/10.3919/ringe1963.56.708

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Abstract

We describe a female patient with TA-GVHD diagnosed by the PCR method. A 64-year-old woman underwent Miles' operation for anal cancer. She was intraoperatively transfused with 400ml of unirradiated conservative blood, including male blood, and this was followed by the onset of fever, diarrhea and a red rash over the entire body after 10th postoperative day. The patient died of multiple organ failure secondary to sepsis on the 21st day after the blood transfusion. She had no background of immunodefficiency. GVHD was diagnosed on the basis of lymphocyte chimerism, HLA-typing, the clinical course, and a skin biopsy. To demonstrate lymphocyte chimerism, we used PCR with primers for the sequences of the sex-related Y gene. We consider this a very simple and useful method of making a diagnosis of GVHD in female patients.

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