One known (1) and two new cycloartane triglycosides, 20S,22R,23S,24R-16β,23;22,25-diepoxy-cycloartane-3β,23,24-triol 3-O-β-D-glucopyranosyl-(1→2)-β-D-glucopyranosyl-(1→2)-β-D-xylopyranoside (2) and 20S,22R,23S,24R-16β,23;22,25-diepoxy-cycloartane-3β,23,24-triol 3-O-(6-O-trans-isoferuloyl-β-D-glucopyranosyl)-(1→2)-β-D-glucopyranosyl-(1→2)-β-D-xylopyranoside (3), were isolated from a commercial Cimicifuga Rhizome. Their structures were determined by two dimensional (2D) NMR spectroscopic analysis and chemical evidence. These compounds suppressed the proliferation of lymphocytes in mouse allogeneic mixed lymphocyte reaction.