Endocrine Journal
Online ISSN : 1348-4540
Print ISSN : 0918-8959
ISSN-L : 0918-8959
ORIGINALS
APS-mediated Ubiquitination of the Insulin Receptor Enhances its Internalization, but does not Induce its Degradation
Kazuhiro KISHIKazuaki MAWATARIKikuko SAKAI-WAKAMATSUTomoyuki YUASAMiao WANGMakoto OGURA-SAWAYutaka NAKAYAShigetsugu HATAKEYAMAYousuke EBINA
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JOURNAL FREE ACCESS

2007 Volume 54 Issue 1 Pages 77-88

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Abstract

APS, a tyrosine kinase adaptor protein with pleckstrin homology and Src homology 2 domains, is rapidly and strongly tyrosine-phosphorylated by insulin receptor kinase upon insulin stimulation. We have previously shown that APS knockout mice have increased insulin sensitivity, and that this enhancement is possibly due to increased insulin-response on adipose tissues. However, the function of APS in insulin signaling has so far been controversial. Here, we report that APS enhanced ligand-dependent multi-ubiquitination of the insulin receptor (IR) in CHO cells overexpressing the IR. APS-mediated ubiquitination of the IR induced enhancement of the IR internalization, but did not affect the IR degradation. This finding shows one of the pleiotropic functions of APS in insulin signaling.

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© The Japan Endocrine Society
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