The Journal of Antibiotics
Online ISSN : 1881-1469
Print ISSN : 0021-8820
ISSN-L : 0021-8820
NEW ANTIBIOTIC-PRODUCING STREPTOMYCETES, SELECTED BY ANTIBIOTIC RESISTANCE AS A MARKER
I. NEW ANTIBIOTIC PRODUCTION GENERATED BY PROTOPLAST FUSION TREATMENT BETWEEN STREPTOMYCES GRISEUS AND S. TENJIMARIENSIS
FUMIO YAMASHITAKUNIMOTO HOTTASHOGO KURASAWAYOSHIRO OKAMIHAMAO UMEZAWA
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1985 Volume 38 Issue 1 Pages 58-63

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Abstract

A novel antibiotic was found after performing an interspecific fusion treatment between Streptomyces griseus and S. tenjimariensis by the selection of clones with a unique antibiotic resistance. Nonantibiotic-producing mutants of streptomycin (SM)-producing S. griseus SS-1198 with resistance to SM and istamycin (IS)-producing S. tenjimariensis SS-939 with resistance to kanamycin (KM) were protoplasted, mixed with polyethyleneglycol and regenerated. Resistant clones to both SM and KM were found among spores of the regenerated culture at a frequency of 10-6. Their growth appearance was identical with that of S. griseus. Antibiotic productivity was found only in clones resistant to both 20-50μg/ml of KM and 400μg/ml of SM. The antibiotic produced by a selected strain, SK2-52, proved to be different from SM and IS.

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© Japan Antibiotics Research Association
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