Characterization of B-5354c, a New Sphingosine Kinase Inhibitor, Produced by a Marine Bacterium
2000 Volume 53 Issue 8 Pages 759-764
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2000 Volume 53 Issue 8 Pages 759-764
B-5354c is a new inhibitor of sphingosine kinase from a novel marine bacterium, SANK 71896. Kinetic study revealed that B-5354c inhibits sphingosine kinase with a Ki value of 12μM. The inhibition is noncompetitive with respect to sphingosine. The compound also inhibits sphingosine-1-phosphate formation in human platelets. Experiments using synthetic derivatives of B-5354c indicate that all the three functional groups, i.e., the long unsaturated aliphatic chain, 4-amino and 3-hydroxyl groups are necessary to inhibit sphingosine kinase.
