The Journal of Biochemistry
Online ISSN : 1756-2651
Print ISSN : 0021-924X
Novel Structure of the N-Acetylgalactosamine Containing N-Glycosidic Carbohydrate Chain of Batroxobin, a Thrombin-Like Snake Venom Enzyme
Nobuhiro TanakaHiroshi NakadaNobuyuki ItohYasuko MizunoMasami TakanishiToshisuke KawasakiShin-ichi TateFuyuhiko InagakiIkuo Yamashina
Author information
JOURNAL FREE ACCESS

1992 Volume 112 Issue 1 Pages 68-74

Details
Abstract

The structure of the Asn-linked carbohydrate chain of batroxobin, a thrombin-like enzyme from Bothrops atrox moojeni snake venom, has been determined. The sugar chain was isolated from batroxobin by hydrazinolysis followed by pyridylamination (PA). The PA-oligosaccharide chain was purified by HPLC on an anion exchange or reverse phase columns, and its structure was examined by sequential exoglycosidase digestion, 600MHz 1H NMR spectroscopy and methylation analysis. The results indicate that the oligosaccharide chain has the following structure involving a novel linkage, NeuAcα2→3GalNAc.
_??_

Content from these authors
© The Japanese Biochemical Society
Previous article Next article
feedback
Top